Experimental instrument

Test tube, Micropipettor, Vortex mixer, 37℃ water bath/gas bath, Spectrophotometer (546nm)

Operation steps

Operate with test tubes. Colorimetric assay with Spectrophotometer.

Performance index

1.  The absorbance of the blank tube is ≦0.050 (Optical path is 0.5cm).

2.  Sensitivity: The absorbance value △A is between 0.180~0.280 when testing 2.6 mmol/L samples.

3.  Linear range: 2~12 mmol/L, r2＞0.995.

4.  Precision: intra CV≤ 8%, inter CV ≤ 10%.

5.  Stability: This kit can be stored at 2~8℃ with shading light for 12 months and can be stable for 1 month since it is opened with the same storage condition.

Notes

1.  This product is for scientific research use only, not for clinical diagnosis. Do not eat.

2.  If the sample content is beyond the maximum limit, please dilute the sample with saline before detection, and multiply the result by the dilution ratio.

3.  Protect the reagent from contamination of glucose, cholesterol, etc.

4.  The amount of reagent and sample can be increased and decreased according to the volume of cuvette.