Application

This kit can be used for detecting the concentration of sodium ions in serum or plasma samples. 

Detection principle

Production of o-nitrophenol and galactose by o-nitrophenol-β-D-galactoside(ONPG) catalyzed by sodium dependent β-D-galactosidase. The amount of o-nitrophenol is directly proportional to the concentration of sodium ion in the sample. The o-nitrophenol is yellow in alkaline environment. The increase of absorbance is determined at 405nm, and the content of sodium ion is calculated indirectly.

Experiment instruments

Spectrophotometer (405 nm), Biochemical analyzer (405 nm), Microplate reader(405 nm), Micropipettor  

Sample requirement

Separate the serum or plasma as soon as possible after blood collection to avoid hemolysis.

Operation steps

1.    Detection with Microplate reader:

2.    Detection with spectrophotometer:

3.    Detection with biochemical analyzer: input the parameters follow the table.

Relevant parameters

1.  Absorbance of blank: A405 ≤ 0.5, △A405 ≤0.4 (optical path = 1 cm)

2.  Linear range: 80~180 μmol/L, |R| ≥0.995.

3.  Sensitivity: (|△A|/min) is between 0.0011 ~ 0.0036 when testing 140 mmol/L samples.

4.  Accuracy: relative deviation ≤ 10%。

5.  Precision: intra-CV≤ 5.0%，inter- CV ≤ 8%.

Notes

1.  This kit is for research use only.

2.  Please operate strictly according to operation procedures.

3.  Do not use expired kit or reagents of different batches.

4.  Please take safety precautions and follow the procedures of laboratory reagent operation. All waste liquid should be handled in accordance with local regulations.