Application

This kit can be used for detection of fructose content in samples, such as juice, honey, seminal plasma, tissues, etc. This kit (50 Assays) can detect 48 samples.

Detection significance

Fructose mainly derives from the secretion of seminal vesicle, which is the primary sugar of seminal fluid and one of the important nutritious components of sperm. There is a prominent correlation between fructose content in semen and sperm activity. The fructose content in semen can influence the sperm activity directly. And it is an important factor to measure whether the activity is good or bad. Moreover, fructose is the sweetest in natural sugar and equal to 1.8 times of sucrose. The composition of saccharides is an important indicator to measure the quality of juice and honey. The establishment of determination method has a great significance for controlling the quality of honey and detecting fructose content in juice.

Detection principle

The product of fructose and matrix solution has a maximum absorption peak at 285 nm. The fructose content can be calculated indirectly by measuring the OD value at 285 nm.

Experimental instruments

Test tube, Micropipettor, Vortex mixer, 100℃ water bath, Spectrophotometer (285 nm)

The preparation of sample

1. Juice: Extract fresh fruit juice, then centrifuge at 3500 rpm for 10 min. Take the supernatant for test.

2. Honey: Dilute the honey with double distilled water for 1000 times.

3. Seminal plasma: Collect fresh semen samples and place at room temperature for 1 hour, then centrifuge at 2500 rpm for 15 min. Take the supernatant for test.

4. Animal tissue: Weigh the tissue accurately and add double distilled water at a ratio of weight (g): volume (mL) =1: 4, homogenize the tissue in ice bath to prepare 20% tissue homogenate, centrifuge at 2500 rpm for 10 min. Take the supernatant for test. Meanwhile, determine the protein concentration of supernatant (E-BC-K318, E-BC-K168, E-BC-K165).

Operation steps

[Note]: For tissue homogenate with high protein concentration, the reaction solution might be cloudy. It is recommended to centrifuge at 4000 rpm for 10 min after cooling and then take the supernatant for measuring the OD values. 

Notes

1. This kit is for research use only.

2. Instructions should be followed strictly, changes of operation may result in unreliable results.

3. The validity of kit is 6 months.

4. Do not use components from different batches of kit.