This kit can be used to measure the sucrase activity in tissue samples. This kit (50 Assays) can detect 25 samples.

Detection significance

Sucrase is one of the key enzymes for carbohydrate digestion and absorption, which can hydrolyze sucrose into the corresponding monosaccharide and be absorbed by the body.

Detection principle

The generated reducing sugar catalyzed by sucrase was determined by 3.5-dinitrosalicylic acid method, and the hydrolysis rate of sucrase can be calculated. The principle is that 3.5-dinitrosalicylic acid and reducing sugar are reduced to brown-red amino compounds. The amount of reducing sugar is proportional to the color depth of the reaction solution within a certain range. This method is easy to operate, fast, less impurity interference.

Experiment instruments

Test tube

Micropipettor  

Water bath

Centrifuge  

Spectrophotometer (520 nm)

Preparation of sample

Add Extract Solution into the tissue sample according to the ratio of Weight (g): Extraction Solution (mL) =1:5~10 (it is recommended to add 1 mL of Extraction Solution into 0.1 g of tissue), then homogenize the sample on ice. Centrifuge the tissue homogenate at 8000 g for 10 min at 4℃. Take the supernatant and preserve it on ice for detection.

Detection procedures

1. Preheat the Spectrophotometer for 30 min, then adjust the wavelength at 520 nm and set to zero with distilled water.

2. Operation table

Note:

1. The kit is for scientific research only.

2. Instructions should be followed strictly, changes of operation may result in unreliable results.

3. The valid of kit is 3 months.

4. Do not use components from different batches of kit.