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상품 상세설명
The Genomic DNA Maxi Kit (Plant) is a genomic DNA extraction maxiprep kit optimized for genomic DNA, mitochondrial DNA and chloroplast DNA purification from plant tissue. This DNA extraction kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the maxiprep spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 50 minutes.
Advantages (Cat. # GPM010, GPM025)
- Purify genomic DNA in less than 50 minutes!
- Binding Capacity: 500 µg
- Sample Size: up to 1 g (fresh plant tissue), up to 250 mg (dry plant tissue)
- Maxiprep Spin Columns: glass fiber membrane optimized for genomic DNA extraction
- Elution Volume: 200 µl ~ 2 ml
- Storage: dry at room temperature (15-25ºC)
Applications
PCR, Real-time PCR, Southern Blotting and RFLP
Components
- GPX1 Buffer (optimized for samples with high polysaccharide content)
- GP1, GP2, GP3 Buffers
- RNase A
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- Filter Column
- GD Maxi Column
- Collection Tube
Quality Control
The quality of the Genomic DNA Maxi Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 500 mg fresh young leaf samples. The purified genomic DNA (>80 µg) is quantified with a spectrophotometer and analyzed by electrophoresis.
Genomic DNA Maxi Kit (Plant) Functional Test Data
 | Figure 1. Genomic DNA (approximately 30 kb) was extracted using the Genomic DNA Maxi Kit (Plant). 0.5 g of fresh lemon leaves and 0.5 g of fresh coffee leaves were ground to a fine powder followed by DNA extraction. 2 µl aliquots from a 1 ml eluate were analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA ladder
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Specifications | ||||||||
| Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK100 GSK300 |
Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Reagent System | Spin Column | Spin Column |
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg |
| Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs |
| 25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||
| 1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||
hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||
| Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A |
| Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes |
| Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | Lyticase or Zymolase | Proteinase K | Proteinase K |
Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10-15 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab |
10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||
| Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 50-100 µl | 30-100 µl | 50-100 µl |
| Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
Product Name | RXNS | Catalogue Number |
100, 300 | GS100/300 | |
100, 300 | GB100/300 | |
10, 25 | GPM010/25 | |
10, 25 | GDM10/25 | |
50, 100, 300 | GT050/100/300 | |
100 | GP100 | |
100 | GMB100/300 | |
100 | GSK100/300 | |
100, 300 | GBYB100/300 | |
100, 300 | GBB100/101/300/301 | |
4 x 96, 10 x 96 | 96GBP04/10 | |
Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 |
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