- Purify genomic DNA in less than 30 minutes!
- High Yield: 3-5 μg (100 mg Thale cress), 20-25 µg (100 mg Tobacco)
- Sample Size: up to 100 mg (fresh plant tissue), up to 25 mg (dry plant tissue)
- Spin Columns: glass fiber membrane optimized for genomic DNA extraction
- Elution Volume: 30-200 µl
- Storage: dry at room temperature (15-25°C)
Applications
PCR, Real-time PCR, Southern Blotting and RFLP
Components
- GPX1 Buffer (optimized for samples with high polysaccharide content)
- GP1, GP2, GP3 Buffers
- RNase A
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- Filter Column
- GD Column
- 2 ml Collection Tubes
Quality Control
The quality of the Genomic DNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 50 mg fresh young leaf samples. The purified genomic DNA (>10 µg) is quantified with a spectrophotometer and checked by electrophoresis.
Genomic DNA Mini Kit (Plant) Interactive Protocol
Genomic DNA Mini Kit (Plant) Functional Test Data
 | Figure 1. Genomic DNA was extracted from a variety of 100 mg plant sample species using the Genomic DNA Mini Kit (Plant). A 3 µl aliquot from each 200 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA ladder 1. Cinnamomun camphora (Camphor tree) 2. Pisum sativum (Pea sprout) 3. Arabidopsis thaliana (Thale cress) 4. Oryza sativa (Rice) 5. Ipomoea batatas (Sweet potato vine) 6. Rhizoma dioscoreae (Chinese yam ) 7. Populus tremula (Aspen) 8. Flammulina velutipes (Mushroom) 9. Oxalis comiculats (Clover) | ||||||||||||||||||||||||
| |||||||||||||||||||||||||
Table 1. The approximate genomic DNA yield from a variety of plant species using the Genomic DNA Mini Kit (Plant). | |||||||||||||||||||||||||
Genomic DNA Extraction Kits Specifications
Specifications | ||||||||
| Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK10 GSK300 |
Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column |
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg |
| Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs |
| 25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||
| 1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||
hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||
| Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A |
| Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes |
| Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | N/A | Proteinase K | Proteinase K |
Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab |
10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||
| Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 30-200 µl | 30-100 µl | 50-100 µl |
| Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
Related Genomic DNA Extraction Products
Product Name | RXNS | Catalogue Number |
100, 300 | GS100/300 | |
100, 300 | GB100/300 | |
10, 25 | GPM010/25 | |
10, 25 | GDM10/25 | |
50, 100, 300 | GT050/100/300 | |
100 | GP100 | |
100 | GMB100/300 | |
100 | GSK100/300 | |
100, 300 | GBYB100/300 | |
100, 300 | GBB100/101/300/301 | |
4 x 96, 10 x 96 | 96GBP04/10 | |
Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 |