The Presto™ Mini gDNA Bacteria Kit is a Bacteria genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells. This Bacteria Genomic DNA Extraction Kit includes Gram+ Buffer and Lysozyme and when combined, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions.
Advantages (Cat. # GBB100, GBB101, GBB300, GBB301)
Quality Control
Figure 1. Genomic DNA (approximately 30 kb) was extracted using the Presto™ Mini Bacteria Kit. An Escherichia coli (1×109) culture (OD600=2, 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. A 5 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
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Figure 2. Genomic DNA (approximately 30 kb) was extracted using the Presto™ Mini Bacteria Kit. A Bacillus subtilis (1×109) culture (OD600=2 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. A 5 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
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Specifications | ||||||||
Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK100 GSK300 |
Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column |
Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg |
Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs |
25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||
1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||
hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||
Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A |
Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes |
Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | N/A | Proteinase K | Proteinase K |
Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab |
10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||
Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 30-200 µl | 30-100 µl | 50-100 µl |
Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
Product Name | RXNS | Catalogue Number |
100, 300 | GS100/300 | |
100, 300 | GB100/300 | |
10, 25 | GPM010/25 | |
10, 25 | GDM10/25 | |
50, 100, 300 | GT050/100/300 | |
100 | GP100 | |
100 | GMB100/300 | |
100 | GSK100/300 | |
100, 300 | GBYB100/300 | |
100, 300 | GBB100/101/300/301 | |
4 x 96, 10 x 96 | 96GBP04/10 | |
Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 |
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