The Presto™ Buccal Swab gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic, mitochondrial and viral DNA) from buccal cells. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with the kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. High quality genomic DNA can be purified in 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 2 μg per buccal swab. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions. 

Advantages (Cat. # GSK100, GSK300)

Presto™ Buccal Swab gDNA Extraction Kit Funtional Test Data

	Figure 1. Genomic DNA from 3 individual cotton buccal swab samples was extracted using the Presto™ Buccal Swab gDNA Extraction Kit. The purified genomic DNA was analyzed by electrophoresis on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder Sample DNA Conc. 260/280 Yield Test 1 52.20 ng/µl 1.86 1.83 µg Test 2 34.80 ng/µl 1.90 1.22 µg Test 3 50.90 ng/µl 1.92 1.78 µg
	Figure 2. The purified genomic DNA was used as a template for amplifying partial human 5S ribosomal DNA (91 bp) by PCR. The PCR products were analyzed on a 0.8% agarose gel.   M = Geneaid 100 bp DNA Ladder

Genomic DNA Extraction Kits Specifications

Specifications	gSYNC™ DNA Extraction Kit	Genomic DNA Mini Kit Blood Cultured Cell	Genomic DNA Mini Kit Tissue	Genomic DNA Mini/Maxi Kit Plant	Presto™ Mini gDNA Bacteria Kit	Presto™ Mini gDNA Yeast Kit	Geneius™ Micro gDNA Extraction Kit	Presto™ Buccal Swab gDNA Extraction Kit
Cat. #	GS100 GS300	GB100 GB300	GT050 GT100 GT300	GP100 GPM010 GPM025	GBB100/101 GBB300/301	GBYB100 GBYB300	GMB100 GMB300	GSK100 GSK300
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg (Mini) 500 µg (Maxi)	50 µg	N/A	50 µg	50 µg
Sample	200 µl blood	300 µl blood	30 mg animal tissue	Mini: 100 mg plant tissue	1x109 bacterial cells	2x108 yeast cells	1-100 µl blood	buccal swabs
	25 mg animal tissue	200 µl buffy coat		Maxi: 1 g plant tissue	200 µl blood		FTA® Cards
	1x107 cultured cells	1x107 cultured cells			1 ml urine, plasma		1 ml urine
	hair	2x108 yeast/fungus					1-100 µl saliva
Micropestle	N/A	N/A	Yes	N/A	N/A	N/A	N/A	N/A
Filter Column	N/A	N/A	N/A	Yes	N/A	N/A	N/A	Yes
Enzyme	Proteinase K	N/A	Proteinase K	N/A	Lysozyme Proteinase K	N/A	Proteinase K	Proteinase K
Typical Yield	5 µg from 200 µl blood	4-10 µg from 300 µl blood	10-40 µg from 20 mg tissue	3-5 µg from 100 mg Arabidopsis leaf (Mini)	37 µg from 1x109 E. coli	10-15 µg from 2x108 S. cerevisiae cells	300 ng from 6 mm blood spots	2 µg per swab
	10-40 µg from 20 mg tissue			80 µg from 500 mg Lemon leaf (Maxi)	10-15 µg from 1x109 Bacillus subtilis
Elution Volume	30-100 µl	30-200 µl	30-200 µl	30-200 µl (Mini) 1 ml (Maxi)	30-200 µl	30-200 µl	30-100 µl	50-100 µl
Operation Time	<20 minutes	<25 minutes	30 minutes	<30 minutes	<30 minutes	<40 minutes	<20 minutes	<20 minutes

Related Genomic DNA Extraction Products

Product Name	RXNS	Catalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)	100, 300	GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)	100, 300	GB100/300
Genomic DNA Maxi Kit (Plant)	10, 25	GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)	10, 25	GDM10/25
Genomic DNA Mini Kit (Tissue)	50, 100, 300	GT050/100/300
Genomic DNA Mini Kit (Plant)	100	GP100
Geneius™ Micro gDNA Extraction Kit	100	GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit	100	GSK100/300
Presto™ Mini gDNA Yeast Kit	100, 300	GBYB100/300
Presto™ Mini gDNA Bacteria Kit	100, 300	GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GPP04/10