Tissue/Blood DNA Mini Kit (GS100, GS300)

The gSYNC™ DNA Extraction Kit is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), serum, plasma, buffy coat, body fluids, cultured cells, tissue, rodent tails, ear punches, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid, hair, insects and sperm in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in less than 20 minutes without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions. 

Advantages (Cat. # GS100, GS300)

• Purify genomic DNA within 20 minutes!
• Variety of Samples: 50-200 μl of whole blood, plasma, serum, buffy coat, up to 25 mg of tissue, rodent tails, ear punches, insects, up to 1 x 107 cultured cells, up to 25 mg of formalin-fixed-paraffin-embedded tissue, up to 15 ml of amniotic fluid, hair, up to 100 µl of sperm
• High Yield: 5 µg from 200 µl whole blood
• High Quality DNA: A260/A280 = 1.8-2.0
• Spin Columns: glass fiber membrane optimized for genomic DNA extraction
• Elution Volume: 30-100 µl
• Storage: dry at room temperature (15-25°C)

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

• GST Buffer
• GSB Buffer
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
• Proteinase K
• GS Columns
• 2 ml Collection Tubes

Quality Control

The quality of the gSYNC™ DNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 200 μl of whole human blood is extracted using the gSYNC™ DNA Extraction Kit. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Figure 1. Genomic DNA from 50, 100 and 200 µl whole blood samples was extracted using the gSYNC™ DNA Extraction Kit. 10 µl from 100 µl eluates of purified genomic DNA was analyzed by electrophoresis on a 0.8% agarose gel. 1-3 = 50 µl whole blood sample, 4-6 = 100 µl whole blood sample, 7-9 = 200 µl whole blood sample M = Geneaid 1 Kb DNA Ladder Sample Yield 260/280 50 µl 1.54 µg 1.85 100 µl 2.70 µg 1.87 200 µl 5.56 µg 1.90