Dry Blood Spot DNA Kit (GMB100, GMB300)

The Geneius™ Micro gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots (Whatman® FTA® Cards), urine and saliva. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with this DNA extraction kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in as little as 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 150-200 ng from 6 mm dried blood spots. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions. 

Advantages (Cat. # GMB100, GMB300)

• Purify genomic DNA from small volume blood samples (1 µl), dried blood spots (Whatman® FTA® Cards), urine, saliva
• Time: in as little as 20 minutes
• High Yield: Up to 300 ng of pure genomic DNA per 6 mm diameter punch from Whatman® FTA® Cards (dried blood spots)
• High Purity gDNA: A260/A280 = 1.8-2.0
• DNA Size: 20-30 kb
• Spin Columns: glass fiber membrane optimized for genomic DNA extraction
• Elution volume: 30-100 µl
• Storage: dry at room temperature (15-25°C)  

Applications

 

PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

 

Components

• S1 Buffer
• S2 Buffer
• Carrier RNA
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• Proteinase K
• GD Columns
• 2 ml Collection Tubes

Quality Control

The quality of the Geneius™ Micro gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (150-200 ng with an A260/A280 ratio of 1.8-2.0) from 10 µl blood samples is quantified with a spectrophotometer and analyzed by electrophoresis.

 

Real-Time PCR Data

Figure 1. Genomic DNA was extracted from blood samples (1–64 µl) using the Geneius™ Micro gDNA Extraction Kit. The purified DNA was eluted in 30 µl of Elution Buffer followed by a Real-time PCR assay performed with a 3 μl aliquot as template, primers (designed to amplify the ACTB gene), and Fast SYBR Green PCR Master Mix using the StepOnePlus™ Real-Time PCR system (Applied Biosystems). The results confirm the efficiency of DNA extraction from as low as 1 µl blood samples.