GENEzol™ Bacteria Kit (GZB050/GZB100/GZB200)

The GENEzol™ TriRNA Bacteria Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from bacteria samples. Bacterial cell walls are initially lysed using Lysozyme. The sample is then homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications. 

Advantages (Cat. # GZB050, GZB100, GZB200)

• Purify total RNA within 20 minutes without chloroform phase separation or isopropanol RNA precipitation
• Sample Volume: bacteria cells up to 1 x 109
• Format: phenol, chlorform, guanidine isothiocyanate plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, Lysozyme can be shipped at room temperature but should be stored at -20ºC for extended periods

• GENEzol™ Reagent
• Pre-Wash Buffer
• Lysozyme
• Bacteria Lysis Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Figure 1. RNA was extracted using the GENEzol™ TriRNA Bacteria Kit. An Escherichia coli (1×109) culture (OD600=2, 1 ml) was harvested by centrifugation at 16,000 x g for 2 minutes, followed by RNA extraction. 5 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder Test RNA Concentration 260/280 260/230 Yield 1 502.6 µg/ml 2.06 2.18 25.13 µg 2 518.3 µg/ml 2.07 2.21 25.92 µg 3 506.0 µg/ml 2.08 2.24 25.30 µg