GENEzol™ Reagent (GZR050/GZR100/GZR200)

GENEzol™ Reagent is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting. GENEzol™ Reagent is a cost effective alternative for efficient total RNA extraction. 

Advantages (Cat. # GZR050, GZR100, GZR200)

• Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
• Sample Volume: up to 300 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 50-100 mg of tissue
• A cost effective, scalable solution
• Format: phenol, chlorform, guanidine isothiocyanate
• Storage: dry at 2ºC to 25ºC

• GENEzol™ Reagent

Figure 1. Bacterial RNA was extracted from 5 x 108 E. coli cells using our top competitor's TRI reagent and GENEzol™ Reagent. The E. coli cells were incubated with lysozyme for 5 minutes at room temperature. 1 ml of reagent was then added to the cell lysate. The RNA pellets were dissolved in 50 μl of RNase-free water. TRI Reagent = 1, 2 GENEzol™ Reagent = 3, 4 Sample ng/µl 260/280 260/230 Yield (µg) 1 241.3 1.77 0.66 12.1 2 260.2 1.83 0.85 13.0 3 315.6 1.78 0.75 15.8 4 293.4 1.81 0.80 14.7

GENEzol™ Reagent Functional Test Data



Figure 2. RNA from a 1 ml human blood sample was extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder Test RNA Conc. 260/280 Yield 1 119.9 µg/ml 1.82 6.0 µg 2 135.5 µg/ml 1.76 6.8 µg 3 176.1 µg/ml 1.77 8.8 µg

	RNA was extracted from 300 µl of human blood (2.8 x 1010 copy/ml) using GENEzol™ Reagent.
	RNA standard (107 copy/ml) RNA standard (106 copy/ml) RNA standard (105 copy/ml)
	RNA standard (104 copy/ml)