The GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications. 

Advantages (Cat. # GZX050, GZX100, GZX200)

• Purify total RNA in 15 minutes!
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• No chlorophorm phase separation
• No isopropanol RNA precipitation
• No phenol carryover
• Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
• Spin Columns: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
• Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, other components should be stored at room temperature (15-25ºC)

• GENEzol™ Reagent
• Pre-Wash Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Figure 1. RNA was purified using the GENEzol™ TriRNA Pure Kit in parallel to the similar product from competitor Z. 5x105 HeLa cells were homogenized using GENEzol™ Reagent and competitor Z tri reagent. RNA was then purified using the corresponing kits spin column procedure. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel. Test RNA Conc. 260/280 260/230 Yield 1. Z 162.5 ng/µl 2.00 2.07 8.1 µg 2. Z 160.7 ng/µl 2.03 2.07 8.0 µg 3. Geneaid 164.0 ng/µl 2.00 2.07 8.2 µg 4. Geneaid 161.6 ng/µl 2.03 2.06 8.0 µg Table 1. Total RNA purified using the GENEzol™ TriRNA Pure Kit and competitor Z.

Test RNA Conc. 260/280 260/230 Yield 1 58.6 ng/µl 1.86 1.81 2.1 µg 2 68.1 ng/µl 1.87 1.81 2.4 µg 3 74.2 ng/µl 1.87 1.83 2.6 µg Table 2. RNA was purified using the GENEzol™ TriRNA Pure Kit. RNA from 200 µl of whole human blood (3 donors) was initally homogenized using GENEzol™ Reagent. RNA was then purified using a spin column procedure. 10 µl from each 35 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.

	RNA was extracted from 200 µl of human blood (5.2 x 1010 copy/ml) using GENEzol™ TriRNA Pure Kit.
	RNA standard (107 copy/ml) RNA standard (106 copy/ml) RNA standard (105 copy/ml)
	RNA standard (104 copy/ml)
	

Figure 3.  Quantitative analysis of human beta globin mRNA purified by GENEzol™ TriRNA Pure Kit using a Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.