miRNA Isolation Kit (RMI050)

The miRNA Isolation Kit provides a quick and easy spin column system for purifying and enriching micro RNAs (miRNAs) and other small cellular RNAs from a wide variety of tissue and cells. Since miRNAs are vital for regulating gene expression, this kit is optimized for isolation of small RNA molecules while removing larger RNAs and minimizing genomic DNA contamination for improved sensitive downstream applications. 

Advantages (Cat. # RMI050)

• Purify miRNA within 30 minutes!
• High yield of miRNA and other small RNA
• Efficient removal of larger RNA for sensitive downstream applications
• Sample: 100 mg of tissue, up to 1 x 106 cultured cells
• Spin Columns: glass fiber membrane optimized for miRNA extraction
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
• Micropestle: certified RNase and DNase-free
• Storage: dry at room temperature (15-25ºC) for up to 9 months

Applications

miRNA analysis, siRNA analysis, shRNA analysis and snRNA analysis, qPCR, microarray analysis

Components

• Lysis Buffer
• Mi Buffer
• Wash Buffer
• Release Buffer
• RNA Columns
• 2 ml Collection Tubes
• Micropestles

Quality Control

The quality of the miRNA Isolation Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 1/5 volume aliquots are analyzed on a polyacrylamide gel. The majority of RNA visible on the gel are <100 nt in size, with the major bands corresponding to tRNAs. The 5S and 5.8S rRNA species may also be visible. These tRNA and small rRNA bands should be clear and distinct. miRNA (21-22 nt) are typically not visible on the gel image.

miRNA Isolation Kit Functional Test Data

	Figure 1. Total RNA from mouse tissue was isolated using a reagent system.10 µg of each RNA was subjected to miRNA isolation using the miRNA Isolation Kit. The purified miRNA was resolved in 50 µl of Release Buffer and a 1/10 volume aliquot (5 µl) was analyzed by electrophoresis on a 2% agarose gel.
	Figure 2. Small RNAs were isolated from 4 human placental samples. Each lane contains 1/10 of the purified miRNA.

Total RNA Extraction Kits Specifications