The PrestoTM FFPE RNA Mini Kit was designed specifically for purifying total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue. Paraffin is initially dissolved with organic solvents such as Xylene followed by Proteinase K digestion. RNA in chaotropic salt is then bound by the glass fiber matrix of the spin column and DNA contamination is removed using an in column DNase treatment. Other contaminants are removed using Wash Buffer and the purified total RNA is eluted in RNase-free Water. High quality total RNA can be purified within 80 minutes without phenol extraction or alcohol precipitation. The purified RNA is ready for use in Real-time PCR, mRNA selection, cDNA synthesis and NGS.
Advantages (Cat. # RFD050, RFD100 )
Quality Control
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Total RNA Extraction Kits Specifications
Specifications | |||||||
Cat. # | RBD050 RBD100 RBD300 | RTD050 RTD100 RTD300 | RPD050 RPD100 RPD300 | RBBD050 RBBD100 RBBD300 | RBYD050 RBYD100 RBYD300 | RMI050 | GZXD050 GZXD100 GZXD200 |
Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Tri Reagent Spin Column |
Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg |
Sample | blood, cells | animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells | blood,cells, tissue, body fluids, bacteria |
Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A |
Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A |
Enzyme | Dnase I | Dnase I | Dnase I | Lysozyme Dnase I | Dnase I | N/A | DNase I |
Typical Yield | 2-3 µg from 300 µl blood, 25-30 µg from 106 cells | 5-30 µg | 5-30 µg | 40-50 µg from 1x109 bacterial cells | 15-20 µg from 5x107 yeast cells | 5-30 µg | 5-30 µg |
Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl |
Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
Related RNA Extraction Products
Product Name | RXNS | Catalogue Number |
50, 100, 300 | RB050/100/300, RBD050/100/300 | |
50, 100, 300 | RT050/100/300, RTD050/100/300 | |
50, 100, 300 | RP050/100/300, RPD050/100/300 | |
50, 100, 300 | RBB050/100/300, RBBD050/100/300 | |
50, 100, 300 | RBY050/100/300, RBYD050/100/300 | |
50 | RMI050 | |
GENEzol™ TriRNA Pure Kit | 50, 100, 200 | GZX050/100/200, GZXD050/100/200 |
GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96, 10 x 96 | 96GZX04/10 |
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