S-Adenosylhomocysteine (SAH) ELISA Kit

• Detection sensitivity limit of 7ng/mL SAH-BSA
• Suitable for use with plasma, serum, lysates, and other biological fluid samples
• SAH-BSA standard included

 

Video: Color Development in an ELISA

Our S-adenosylhomocysteine (SAH) ELISA is designed for detection and quantitation of SAH in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAH antibody are added to a plate coated with a SAH conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve.  Samples with high SAH levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAH levels will leave more antibody available to bind to the conjugate, producing a high signal.