Homocysteine ELISA Kit

• Detection sensitivity limit of 10ng/mL Homocysteine-BSA
• Suitable for use with plasma, serum, lysates, and other biological fluid samples
• Homocysteine-BSA standard included

 

Video: Color Development in an ELISA

Our Homocysteine ELISA is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-homocysteine antibody are added to a plate coated with a homocysteine conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve.  Samples with high homocysteine levels will bind to most of the antibody, which gets washed away and results in a low OD, while samples with low homocysteine levels will leave more antibody available to bind to the conjugate, producing a high signal.