[E-EL-H0747] Human CD109(Cluster of Differentiation 109) ELISA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사 Elabscience
납기일 1-2 주
판매가격 852,600원
포인트 0점(구매금액 : 0%)
배송비결제 3,500원   (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

  • [E-EL-H0747] Human CD109(Cluster of Differentiation 109) ELISA Kit (+0원)

상품 정보

상품 기본설명

96T

상품 상세설명

 

Intended use


This ELISA kit applies to the in vitro quantitative determination of Human CD109 concentrations in serum, plasma and other biological fluids.

Test principle


This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD109. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD109 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD109, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD109. You can calculate the concentration of Human CD109 in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time3.5h
ReactivityHuman
Detection MethodColormetric
Detection Range0.31—20 ng/mL
Sensitivity0.19 ng/mL
Sample Volume Required Per Well100μL
Sample TypeSerum, plasma and other biological fluids

Specificity


This kit recognizes Human CD109 in samples. No significant cross-reactivity or interference between Human CD109 and analogues was observed.

Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(ng/mL)
O.DAverageCorrected
202.521
2.547
2.5342.454
101.729
1.753
1.7411.661
50.989
0.987
0.9880.908
2.50.507
0.521
0.5140.434
1.250.269
0.269
0.2690.189
0.630.196
0.174
0.1850.105
0.310.127
0.139
0.1330.053
00.071
0.089
0.08--
SC-E-EL-H0747.jpg

 

 

 

 

 

 

 

 

 

 

Precision

 

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CD109 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CD109 were tested on 3 different plates, 20 replicates in each plate.

 Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)1.093.138.161.022.938.36
Standard deviation0.060.160.430.060.130.44
C V (%)5.505.115.275.884.445.26

Recovery


The recovery of Human CD109 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)93-110100
EDTA plasma (n=5)83-9590
Cell culture media (n=5)89-10596

Linearity


Samples were spiked with high concentrations of Human CD109 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

  Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)98-11388-10498-114
Average (%)10395106
1:4Range (%)86-9984-9689-99
Average (%)938994
1:8Range (%)92-10781-9287-100
Average (%)998792
1:16Range (%)91-10384-9686-100
Average (%)989193

Kit components & Storage


An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20°C, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20°C(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8°C(shading light)
Stop Solution1 vial, 10 mL2-8°C
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required


  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37°C
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay Procedure



elisa assay procedure 1 

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

 

상품문의

등록된 상품문의

상품문의가 없습니다.

반품/교환정보

셀젠바이오샵에서는 다음과 같은 기간 및 내용으로 상품에 대하여 교환, 반품, 환불을 보장하고 있으며, 상품의 반환에 의한

추가비용을 고객에게 부담시지키 않습니다. (, 고객 변심 또는 주문 반복으로 인한 경우의 반환비용은 고객님이 부담하셔야

합니다.)

 

::: 교환 및 반품이 가능한 경우:::

-상품을 공급 받으신 날로부터 7일 이내

  , 상품을 개봉하여 상품가치가 상실된 경우에는 교환/반품이 불가능합니다.

-공급받으신 상품 및 용역의 내용이 표시, 광고 내용과 다르거나 다르게 이행된 경우
-

:::교환 및 반품이 불가능한 경우:::

-하자가 없는 상품을 택배사로부터 인도 받으신 날로부터 20일 이후.
- 고객님의 책임 있는 사유로 상품등이 멸실 또는 훼손된 경우.
- 포장을 개봉하였거나 포장이 훼손되어 상품가치가 현저히 감소한 경우
- 시간의 경과에 의하여 재판매가 곤란할 정도로 상품등의 가치가 현저히 감소한 경우
- 주문 당시 해외 발주 상품의 경우
-

주문 취소 및 반품으로 환불을 요청하실 경우에는 E-mail(celgen-bio@celgen-bio.com)이나 고객만족센터 (042-824-9026)

통해 요청하시면 친절하게 처리해 드리겠습니다.

주문 취소 후 반품 가능 여부를 확인한 다음 3일 이내에 결제 금액을 환불해 드리겠습니다.
  • ㈜셀젠바이오테크, 대표자명: 김성빈, 사업자등록번호: 344-08-00018, 통신판매업종 제 2017-대전유성-0220 호
  • 대전광역시 대덕구 대화로69, 3층(대화동)
  • 대표전화: 042-824-9026 팩스: 042-824-9027
  • E-Mail: celgen-bio@celgen-bio.com
  • Copyright © 2015 Celgen-Bio.tech All rights reserved.