[E-EL-H0752] Human CD4(Cluster of Differentiation 4) ELISA Kit 요약정보 및 구매


상품 선택옵션 0 개, 추가옵션 0 개

제조사 Elabscience
납기일 2-3 일
판매가격 852,600원
포인트 0점(구매금액 : 0%)
배송비결제 3,500원   (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

  • [E-EL-H0752] Human CD4(Cluster of Differentiation 4) ELISA Kit (+0원)

상품 정보

상품 기본설명


상품 상세설명


Intended use

This ELISA kit applies to the in vitro quantitative determination of Human CD4 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD4. You can calculate the concentration of Human CD4 in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Assay time3.5h
Detection MethodColormetric
Detection Range0.31—20 ng/mL
Sensitivity0.19 ng/mL
Sample Volume Required Per Well100μL
Sample TypeSerum, plasma and other biological fluids


This kit recognizes Human CD4 in samples. No significant cross-reactivity or interference between Human CD4 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.















Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CD4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CD4 were tested on 3 different plates, 20 replicates in each plate.

 Intra-assay PrecisionInter-assay Precision
Mean (ng/mL)0.991.699.320.941.818.91
Standard deviation0.060.080.310.050.090.37
C V (%)6.064.733.335.324.974.15


The recovery of Human CD4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)93-108100
EDTA plasma (n=5)90-10797
Cell culture media (n=5)91-10597


Samples were spiked with high concentrations of Human CD4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

  Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)94-11086-9988-102
Average (%)1029195
1:4Range (%)90-10388-9885-101
Average (%)969392
1:8Range (%)89-10186-9688-101
Average (%)959193
1:16Range (%)93-11083-9587-102
Average (%)1009093

Kit components & Storage

An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Micro ELISA Plate(Dismountable)8 wells ×12 strips-20°C, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20°C(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8°C(shading light)
Stop Solution1 vial, 10 mL2-8°C
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37°C
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay Procedure

elisa assay procedure 1 

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Serum concentrations of CD4+ and CD8+ in patients infected with scabies caused by Sarcoptes scabiei.
    IF: /
    Journal: Journal of Advanced Pharmacy Education & Research| Jan-Mar(2018)
    Products: E-EL-H0752 
    Sample type: Serum
    Reactivity: Human



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