Product introduction
SP-6FF is suitable for separation and purification of all types of charged biomolecules, such as proteins, peptides, nucleic acids, etc.
Advantages
1. Quick, easy, convenient.
2. Wide application range. It is suitable for component separation or fine purification of all types of charged biomolecules.
3. The purification process is highly flexible, and the purity of the sample can be improved by screening the purification conditions in earlier stage.
4. High binding capacity (compared with other types of chromatographic media).
Performance index
Ligand | Sulphopropyl |
Matrix | Highly cross-linked 6% agarose |
Particle size range | 45-165 µm |
Average particle size | 90 µm |
Media type | Strong cation exchanger |
Charged group | -(CH2)3SO3- |
Ionic capacity | 180-300 µmol H+/mL (media) |
Binding capacity | 100 mg Lysyme /mL (media) |
pH stability | 3-14 (short term) 4-13 (working) 4-13 (long term) |
Chemical stability | All of the commonly used buffers, 1M NaOH, 70% Ethanol, 8M Urea, 6M Guanidine hydrochloride. Avoid of oxidizing agent, cationic detergents and cationic buffer |
Flow rate | 200-300 cm/h (0.3MPa, XK16/40. Column bed height: 30 cm) |
Pressure | ≤0.3 MPa |
Storage buffer | 0.2M NaAc, 20% Ethanol |
Storage temperature | 4~30℃ |
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