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Product introduction
G75 (medium) is a kind of gel filtration chromatographic medium (also known as size exclusion chromatography), it is suitable for desalination of biomolecules, buffer exchange, group separation and fine purification.
Advantages
1. Classical glucan medium with good selectivity.
2. Rapid desalination of biological macromolecules (>80kDa) and buffer replacement (one-step).
3. Fast flow rate. It can be used for group separation and removal of impurities (with the exclusion limit of 80 kDa as the cut-off point).
Performance index
Matrix | Cross-linked dextran |
Particle size range (dry powder) | 45-165 µm |
Swelling ratio | 12-15 mL/g |
Particle size range (wet gel) | 80-300 µm |
Separation range | 3-80kDa (Globular proteins) /1-50kDa (Dextran) |
Exclusion limit | 80kDa |
pH stability | 2-10 (long term) 2-10 (working) 2-13 (short term) |
Chemical stability | All commonly used buffers. 8M Urea, 6M Guanidine hydrochloride, all ionic or nonionic detergents, ≤25% Methanol/ Ethanol/ Propyl alcohol (V/V). Avoid of extreme pH (˂2 or ˃13) and oxidant. |
Flow rate | ≥40 cm/h (Inter diameter: 1.6 cm, Column bed height: 30 cm) |
Pressure | ≤0.3 MPa |
Autoclaving | 120℃×30min(wet gel, pH 7.0) |
Storage buffer | 20% Ethanol (for swelling media) |
Storage temperature | 4~30℃ |
Selection of G75
1. Selection of medium grade
(1) Rapid desalination, buffer replacement, group separation, and impurity removal: G75 moderate, large particle diameter and high flow rate.
(2) Separation and purification of small molecular proteins: G75 superfine, slow flow rate and high resolution.
2. Selection of chromatographic column:
(1) Rapid desalination, buffer replacement, group separation, and impurity removal (the sample amount can reach 30% CV): Coarse chromatography column (low column height, e.g., XK 16/60), high flow rate and short cycle.
(2) Separation and purification of small molecular proteins (the sample amount is 0.5%~4% CV): Slender chromatography column (high column height, e.g., XK 16/60), high column efficiency and high resolution.
Application example
Small molecular proteins purified with G75 (super-fine)
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