Application

This kit is used for in vitro quantitative determination of zinc ion (Zn2+) in serum, plasma, urine, etc.

Detection principle

The zinc ion in the sample forms blue complex with zinc reagent at room temperature. Measure the absorption value at 630 nm and calculate the content of zinc. 

Experimental instrument

automatic biochemical analyzer or semi-automatic biochemical analyzer

Sample preparation

The kit is suitable for detecting serum, heparin anticoagulated plasma, urine and semen. Avoid hemolysis. 

Operation steps

1) Main performance parameters

2) Operation table

Technical parameter

1.  Absorbance for the blank control: A630 (1.0 cm)≤0.2.

2. Detection range: 0~30 µmol/L, r2≥0.995.

3. Accuracy: relative deviation≤15.0%.

4. Precision: the intra-assay CV≤4.0% and the inter-assay CV≤6.0%.

5. Sensitivity: for 15.4 µmol/L sample, the absorbance is 0.1-0.2.

1. The kit is for scientific research only. 

2. Instructions should be followed strictly, changes of operation may result in unreliable results. 

3. The validity of kit is 12 months.

4. Do not use components from different batches of kit.

5. Do not use hemolytic serum samples. 

6. As the concentration of Zn2+ in serum is very low, avoid zinc contamination of vessels and reagents used in the experiment.

7. The ratio of sample and reagent can be scaled as required.

8. The sample needs to be diluted with deionized water before determination once the concentration is beyond the linear range. The result should be multiplied by the dilution factor.

9. Choose a filter with the closest wavelength to the required one if there is no suitable filter at hand.

10. No sample deproteinization and sample blanks is required for this method.

11. If the human body surface such as skin, eyes, etc., is contaminated by the reagent, it must be rinsed with clean water. And in case of accidental ingestion, seek medical treatment.

12. The product does not contain poisonous or biological hazardous material.