The Presto™ Urine DNA Extraction Kit is designed for rapid isolation of high-quality cellular and cell-free DNA from up to 5 ml of urine. The kit includes uniquely designed Column Extension Tubes which allow for increased sample volume and yield. Urine samples are lysed using Proteinase K and a buffer containing chaotropic salt. The lysate is mixed with a binding buffer to facilitate DNA binding to the column membrane. The column is then washed and DNA is eluted with Elution Buffer. The entire procedure can be completed within 60 minutes and the purified DNA is ready for use in a variety of downstream applications such as qPCR, Next-Generation sequencing and DNA methylation analysis.  

Specifications

• Operation time: Purify cellular and cell-free DNA within 60 minutes.
• Sample: 1-5 ml of urine
• Format:  Spin columns combined with column extension tubes using vacuum or centrifuge
• Yield: 1-20 ng of DNA per ml of male urine. 5-1000 ng of DNA per ml of female urine.
• Elution Volume: 30-50 µl
• Storage: dry at room temperature (20~25ºC)

Applications

qPCR, Next-Generation sequencing and DNA methylation analysis

Caution

During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

Components

• UR1 Buffer
• UR2 Buffer
• Proteinase K
• W1 Buffer
• Wash Buffer
• Elution Buffer
• Carrier RNA
• Column Extender
• GD Column
• 2 ml Collection Tube

Quality Control

Figure 1. Cellular and cell-free DNA was effectively purified from 5 ml of healthy female urine using Presto™ Urine DNA Extraction Kit.  The purified DNA was eluted with 30 µl Elution Buffer. Purified DNA was analyzed using the Qsep100 capillary electrophoresis system (Bioptic).

Figure 2. Total genomic DNA was isolated from 2, 5 ml of female urine samples (2 replicates of each sample) using PrestoTM Urine DNA Extraction Kit.  The purified DNA was eluted with 30 μl Elution Buffer. A real-time PCR assay was then performed with 1 μl of purified DNA as template, primers (designed to amplify the human GAPDH gene), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The red line corresponds to the purified DNA from 5 ml of urine samples, the green line corresponds to the purified DNA from 2 ml of urine samples. The black line corresponds to the No Template Control.