The Presto™ 96 Well Blood Genomic DNA Extraction Kit was designed for high-throughput purification of genomic, mitochondrial and virus DNA from whole blood (fresh blood and frozen blood), plasma, serum, body fluids, lymphocytes and cultured cells. This DNA extraction kit uses protease and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the Presto™ gDNA 96 Well Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The procedure can be completed within 45 minutes without phenol/chloroform extraction or alcohol precipitation. The purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
Advantages (Cat. # 96GBP04, 96GBP10)
Applications
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
Components
Quality Control
Figure 1. Genomic DNA was extracted from 200 μl whole human blood samples using the Presto™ 96 Well Genomic DNA Extraction Kit. The purified genomic DNA was eluted in 200 µl of Elution Buffer and 15 µl aliquots of the final sample (chosen from 4 random wells) were analyzed by electrophoresis on a 1% agarose gel. M = Geneaid™ 1 Kb DNA Ladder
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Product Name | RXNS | Catalogue Number |
100, 300 | GS100/300 | |
100, 300 | GB100/300 | |
10, 25 | GPM010/25 | |
10, 25 | GDM10/25 | |
50, 100, 300 | GT050/100/300 | |
100 | GP100 | |
100 | GMB100/300 | |
100 | GSK100/300 | |
100, 300 | GBYB100/300 | |
100, 300 | GBB100/101/300/301 | |
4 x 96, 10 x 96 | 96GBP04/10 | |
Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 |
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