The Presto™ Mini gDNA Yeast Kit was designed for rapid isolation of genomic DNA from cultured yeast and other fungus species. The yeast/fungus cells are directly added into beadbeating tubes containing ceramic beads and lysis buffer. Samples are efficiently lysed without the use of degestive enzymes (such as Lyticase or Zymolase) using a bead beating instrument or a standard vortex. The cell lysate is then mixed with Binding Buffer and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.  

Advantages (Cat. # GBYB100, GBYB300)

• Purify genomic DNA from (up to 2 x 108) yeast and other fungus species
• Genomic DNA Yield: up to 10 µg of genomic DNA from 2 x 108 Saccharomyces cerevisiae 
• Format: beadbeating tubes and spin columns
• Elution volume: 30-200 µl 
• Time: 40 minutes
• Storage: dry at room temperature (15-25°C)

Applications

PCR, restricion enzyme digestion and sequencing reactions

Components

• GT Buffer 
• PR Buffer
• GB Buffer
• W1 Buffer
• Wash Buffer
• Elution Buffer
• RNase A (50 mg/m)
• GD Columns
• Beadbeating Tubes Type B
• 2 ml Collection Tubes

Quality Control

Geneaid™ Yeast DNA Extraction Kit Funtional Test Data

Figure 1. Genomic DNA was extracted using the Presto™ Mini gDNA Yeast DNA Extraction Kit. Saccharomyces cerevisiae (50 mg, 100 mg, 200 mg) was harvested by centrifugation at 5,000 x g for 10 minutes. 5 µl aliquots of purified genomic DNA from a 100 µl eluate were analyzed by electrophoresis on a 1% agarose gel.  M = Geneaid 1 Kb DNA Ladder Test DNA Conc. 260/280 260/230 Yield 1 21.2 ng/µl 1.89 2.09 2.1 µg 2 22.2 ng/µl 1.90 2.05 2.2 µg 3 37.5 ng/µl 1.91 2.18 3.8 µg 4 40.7 ng/µl 1.90 2.05 4.1 µg 5 75.3 ng/µl 1.86 2.07 7.5 µg 6 84.9 ng/µl 1.87 2.11 8.5 µg