96 Well Gel Extraction Kit (96DFG04, 96DFG10)

Presto™ 96 Well Gel Extraction Kits were designed to recover or concentrate DNA fragments from agarose gel. QG Buffer (yellow color indicating optimal pH≤7.5) is premixed with a pH indicator to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0) which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes while DNA fragments are bound by the glass fiber matrix of the Presto™ Gel Extraction 96 Well Binding Plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. 

Advantages (Cat. # 96DFG04, 96DFG10)

• Purify up to 300 mg of agarose gel slices per well in 45 minutes!
• High Recovery: up to 80%
• Format: Presto™ Gel Extraction 96 Well Binding Plates
• Broad Fragment Size Range: 70 bp–20 kb
• Centrifuge or vacuum manifold protocol
• Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
• Storage: dry at room temperature (15-25ºC)

Applications

PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation

Components

• QG Buffer (premixed with pH indicator)
• 3M Sodium Acetate (pH5.0)
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
• Presto™ Gel Extraction 96 Well Binding Plates
• 0.35 ml Collection Plates
• Adhesive Film

Quality Control

The quality of the Presto™ 96 Well Gel Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments of various sizes are purified from agarose gel and analyzed by electrophoresis.

Presto™ 96 Well Gel Extraction Kit Functional Test Data

			Figure 1. Gel slice DNA fragments ranging from 250 bp-10 kb were extracted using the Presto™ 96 Well Gel Extraction Kit (lane 1, 2, 3, 4). The purified DNA from a 60 µl eluate (chosen from 4 random wells) was analyzed by electrophoresis on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1100 ng) Test DNA Conc. Yield Recovery 1 14 ng/µl 840 ng  76.4% 2 13.9 ng/µl 834 ng 75.8% 3 13.4 ng/µl 804 ng 73.1% 4 14.6 ng/µl 876 ng 79.6%
Optimal pH        pH Too High		Figure 2. A pH indicator is premixed with QG Buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow.