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96 Well PCR Cleanup Kit (96DFH04, 96DFH10)
Presto™ 96 Well PCR Cleanup Kits were designed for DNA fragments purification from PCR or other enzymatic reactions using an efficient 96 well binding plate system. This PCR Purification Kit uses chaotropic salt to denature enzymes, allowing DNA fragments to be bound by the glass fiber matrix in each well of the 96 well binding plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. The Presto™ 96 Well PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.
Advantages (Cat. # 96DFH04, 96DFH10)
- Purify up to 100 µl of PCR products per well in 20 minutes!
- High Recovery: up to 95%
- Format: Presto™ PCR Cleanup 96 Well Binding Plates
- Broad Fragment Size Range: 70 bp–20 kb
- Centrifuge or vacuum manifold protocol
- Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
- Storage: dry at room temperature (15-25ºC)
Applications
PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation
Components
- Binding Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- Presto™ PCR Cleanup 96 Well Binding Plates
- 0.35 ml Collection Plates
- Adhesive Film
Quality Control
The quality of the Presto™ 96 Well PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments of various sizes are purified from PCR products and analyzed by electrophoresis.
Presto™ 96 Well PCR Cleanup Kit Interactive Protocol
Presto™ 96 Well PCR Cleanup Kit Sequencing Data
Gel Extraction and PCR Cleanup Kits Specifications
Specifications | |||||||
| Cat. # | DFG100 DFG300 | DFC100 DFC300 | DFH100 DFH300 | DFM010 DFM025 | 96DFH04 96DFH10 | DF101 DF301 | DFL100 DFL300 |
Format | Spin Column | Spin Column | Spin Column | Maxi Spin Column | 96 Well Plate | Spin Column | Silica Matrix |
| Binding Capacity | 10 µg | 10 µg | 10 µg | 200 µg | 10 µg | 10 µg | 5 µg |
| Sample | Agarose Gel Slice | PCR Product | Agarose Gel Slice/PCR Product | Agarose Gel Slice/Restriction Enzyme Digest Product | PCR Product | Agarose Gel Slice/PCR Product | Agarose Gel Slice/PCR Product |
| Fragment Size | 70 bp-20 kb | 70 bp-20 kb | 70 bp-20 kb | 100 bp-10 kb | 70 bp-20 kb | 40-200 bp | 100 bp-50 kb |
| pH Indicator | Yes | Yes | Yes | Yes | N/A | N/A | Yes |
| Recovery | Up to 90% | Up to 95% | Up to 95% | Up to 80% | Up to 95% | Up to 95% | Up to 95% (<10 kb) Up to 80% (10-50 kb) |
| Elution Volume | 20-50 µl | 20-50 µl | 20-50 µl | 500-1000 µl | 60-80 µl | 20-50 µl | 10-20 µl |
| Operation Time | 20 minutes | 10 minutes | <20 minutes | <25 minutes | 20 minutes | <20 minutes | <20 minutes |
Product Name | RXNS | Catalogue Number |
100, 300 | DFG100/300 | |
100, 300 | DFC100/300 | |
100, 300 | DFH100/300 | |
10, 25 | DFM010/25 | |
4 x 96, 10 x 96 | 96DFH04/10 | |
100, 300 | DF101/301 | |
100, 300 | DFL100/300 | |
100, 300 | DF100/300 | |
| Presto™ 96 Well Gel Extraction Kit | 4 x 96, 10 x 96 | 96DFG04/10 |
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