Hydrogen Sulfide Gas Assay

• Suitable for use with samples containing a hydrogen sulfide donor, such as enzymes, cells or tissues
• Sodium sulfide standard included

Our Free Hydrogen Sulfide Gas Assay Kit Assay Kit measures free H2S gas emitted from samples. A solution containing silver ions is coated on the underside of a microplate lid to form a thin polymer layer. The lid is placed over a plate containing hydrogen sulfide gas-producing samples. The gas escapes from the liquid sample and makes contact with the polymer in the lid, forming silver sulfide nanoparticles that produce a brown discoloration of the polymer layer. The lid is then moved to an empty microplate where optical density is measured at 405 nm using a standard plate reader.