Ethenoadenosine ELISA

• Detect as little as 50 nM of ethenoadenosine
• Suitable for use with DNA isolated from cells or tissues
• Unknown samples are compared with a provided ethenoadenosine standard

 

Video: Color Development in an ELISA

The OxiSelect™ Aldehyde-Induced DNA Damage ELISA Kit (Ethenoadenosine Quantitation) is a competitive ELISA kit allowing the quantitation of ethenoadenosine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. An anti-ethenoadenosine antibody is added and binds competitively to ethenoadenosine in the unknown sample or to the ethenoadenosine in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of ethenoadenosine in the sample.