S-Glutathione Protein Adduct ELISA

• Quantify s-Glutathione protein adducts in a competitive ELISA format
• Suitable for use with protein samples from cells, lysates, plasma or serum
• Read results in a standard 96-well plate

 

Video: Color Development in an ELISA

The OxiSelect™ s-Glutathione Adduct Competitive ELISA Kit quantifies the glutathionylation of proteins in a standard 96-well plate. Protein standards and unknown samples are added to a plate coated with an s-Glutathione protein conjugate. A primary anti-s-glutathione antibody is added and competes for binding between the s-glutathione adduct in the sample and the s-glutathione adduct bound to the plate. High concentrations of s-glutathione adduct in samples leave little antibody to bind to the plate, resulting in a low OD. When little to no s-glutathione adduct appears in samples, most antibody binds to the plate resulting in a high signal.