Myeloperoxidase Chlorination Activity Assay, Colorimetric

• Quantifies MPO chlorination activity from whole neutrophils, neutrophil lysates, tissue homogenates, or EDTA-plasma samples
• Read results in a standard 96-well colorimetric plate reader
• Quantify unknown samples against a chromogen standard

The OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, colorimetric assay for measuring the myeloperoxidase activity within a sample.  The MPO enzyme catalyzes the reaction of hydrogen peroxide (H2O2) with chloride ions to create hypochlorous acid (HOCl), which rapidly reacts with taurine to produce a stable taurinechloramine product.  This step readily neutralizes the HOCl, which would otherwise accumulate and inactivate MPO.  A catalase-containing stop solution is added to stop MPO catalysis by eliminating hydrogen peroxide.  Finally, taurine chloramine reacts with the yellow TNB chromogen probe, with a decrease in color indicating higher MPOactivity.