6-4PP ELISA for Intact Cells

DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The CPD levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for CPD.

DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The 6-4PP levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for 6-4PP.

DNA Damage Induced by UV Light in Hela Cells. HeLa cells were seeded at 20K/well overnight, then exposed to light under a germicidal lamp for 30 minutes. Immunofluorescence staining of CPD damage was determined as described in the Assay Protocol for #STA-327.