Overview

The E.Z.N.A.® Total RNA Kit I provides a straightforward and rapid method for the isolation of up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues. Multiple samples of up to 1 x 10⁷ eukaryotic cells or 30 mg of tissue can be processed in parallel in fewer than 20 minutes. Purified RNA can be used in many downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.

• Rapid – 20 minutes or less
• Safe – No phenol/chloroform extractions
• Versatile – Spin and vacuum formats available
• High quality – Purified RNA suitable for a variety of downstream applications

Specifications

For Research Use Only. Not for use in diagnostic procedures.

자료보기

Product Data

Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I.

Figure 1. Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I. 

RNA yields from HEK-293 cells using E.Z.N.A.® Total RNA Kit I.

Figure 2. Total RNA was isolated from 2.5×106 HEK-293 cultured cells with Omega Bio-tek’s E.Z.N.A.® Total RNA Kit I with an elution volume of 50 µL. RNA concentrations were quantified with Thermo Scientific’s NanoDrop® 2000c. RNA quality was assessed with the Agilent TapeStation® 2200.