Alpha 1 antitrypsin (A1AT) is a protease inhibitor that belongs to the serine protease inhibitor superfamily. It is categorized as a serum trypsin inhibitor but inhibits a wide range of proteases. A1AT protects against tissue damage resulting from neutrophil elastase expressed by inflammatory cells. When A1AT is not expressed or inactive, neutrophil elastase breaks down elastin, leading to respiratory problems such as emphysema, chronic obstructive pulmonary disease (COPD), and cirrhosis of the liver. A1AT has also been linked to hypercholesterolemia, asthma, granulomatosis with polyangiitis, pancreatitis, gallstones, bronchiectasis and pelvic organ prolapse.
Our Human Alpha 1 Antitrypsin ELISA Kit is a sandwich ELISA for the detection of human alpha 1 antitrypsin in plasma, serum, lysates, and other biological fluids. Samples or standards are added to an anti-A1AT antibody coated plate followed by the addition of a biotinylated anti-human A1AT antibody. A streptavidin enzyme conjugate is then added for detection. The absorbance is read at 450nm and the level of A1AT in samples is determined based on the provided human A1AT standard curve.
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