Phosphatidylglycerol/Cardiolipin Assay Kit

• Detects phosphatidylglycerol and cardiolipin in cell lysate samples
• Detection sensitivity of approximately 1 µM
• Cardiolipin standard curve included

Our Phosphatidylglycerol/Cardiolipin Assay Kit measures both phosphatidylglycerol and cardiolipin in cell lysatesamples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidylglycerol and cardiolipin to glycerol, which is then phosphorlyated by glycerol kinase to yield glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidylglycerol and cardiolipinlevels in unknown sampled are determined based on the provided cardiolipin standard curve.