Overview

The Mag-Bind® PX Blood RNA 96 Kit provides a convenient and fast RNA extraction solution for samples stabilized in PreAnalytiX PAXgene™ or Applied Biosystems’ Tempus™ blood RNA tubes. This system combines Mag-Bind® particles with HiBind® silica technologies for purification of total RNA from up to 2.5 mL preserved blood samples.

The blood sample is spun down and the crude RNA/DNA pellet is collected and washed. The pellet is then resuspended and digested with Proteinase K. The lysate is applied to a filter plate to remove genomic DNA. This procedure completely removes inhibitors and sample stabilization reagents to allow for reliable downstream analysis. High quality purified RNA can be used for downstream applications such as qRT-PCR, RT-PCR, and microarray analysis.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Product Data

Mag-Bind® PX Blood RNA 96 kit competes favorable against a leading competitor in terms of RNA quality and yield.

Figure 1. RNA Integrity Assessment. The quality of the RNA purified was assessed by the RNA Integrity Score (RINe) determined from the electrophoretic trace and Agilent’s TapeStation® 2200 system. 

Omega Bio-tek Mag-Bind PX Blood RNA Kit Electropherogram

Figure 2. Electropherogram Representing Purified RNA. 2.5 mL whole blood was drawn to PAXgene™ blood RNA tubes and stored at room temperature for 24 hours. RNA was purified following manufacturer’s recommended protocols. RNA was analyzed on Agilent’s TapeStation® 2200. 

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