Overview

The Mag-Bind® Total RNA 96 Kit provides a novel technology for total RNA isolation. This kit allows the rapid and reliable isolation of high-quality total cellular RNA and viral RNA from a wide variety of cells and tissues. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, which improves the removal of contaminants and increases nucleic acid purity. Mag-Bind® Total RNA 96 Kit procedure can be fully automated with most robotic workstations.

Protocols are available for the following automated platforms:

• Hamilton Microlab® STAR
• Hamilton Microlab® NIMBUS
• KingFisher™, BioSprint®, and MagMAX® 96

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Product Data

RNA purified using Mag-Bind® Total RNA 96 Kit was of high quality

Figure 1. RNA Integrity. Total RNA was extracted from 1×10⁶ cells using the Mag-Bind® Total RNA Kit. The RNA samples were analyzed with RNA tape on Agilent’s TapeStation® 2200. 

Yield Comparison vs Leading Competitor

Figure 2. RNA Yield Comparison. RNA was isolated using the Mag-Bind® Total RNA Kit and a comparable column-based kit from Company Q. Elution volumes for company Q were 50 µL; for Omega were 100 µL,  RNA was quantified using Thermo Scientific’s NanoDrop® 2000c 

RNA Yield From Mouse Liver

Figure 3. 10 mg mouse liver was isolated with the Mag-Bind Total RNA Kit according to protocol. Total RNA concentration determined by optical density measurements using Thermo Scientific’s NanoDrop® 2000c. Total elution volume was 100 µL 

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