Overview

The Mag-Bind® Universal Pathogen 96 Kit allows rapid and reliable isolation of high-quality host genomic DNA, gram-positive and negative bacterial DNA, fungal spore DNA, viral DNA and viral RNA from tissue, urine, serum, and fecal samples.

The Mag-Bind® Universal Pathogen 96 Kit has a mechanical homogenization step using ceramics beads ranging from 0.1 mm to 2.7 mm to effectively lyse a variety of pathogens. The formulated lysis buffers do not foam during lysis allowing for better homogenization of samples.

The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments. The system combines the Mag-Bind® technology with RBB Buffer to eliminate PCR inhibiting compounds within the samples. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. There are no organic extractions, thus reducing plastic waste and hands-on time and multiple samples can be processed in parallel. 

Product Data

Detection of Fungal Spores

Figure 1. Cryptosporidium oocysts were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific for the target organism. Average of triplicate data shown. 

Detection of Gram-Positive Bacteria

Figure 2. Group B strep cultured samples were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.

Detection of Gram-Negative Bacteria

Figure 3. E. coli cells were cultured overnight in LB broth and added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.

Detection of Viral DNA

Figure 4. HBV viruses were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.

Detection of Viral RNA

Figure 5. Influenza A/B virus was added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.

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