Overview

The Mag-Bind® Blood DNA HV kit is designed for rapid and reliable isolation of high-quality genomic DNA from up to 10 mL whole blood and saliva. The protocol has been successfully automated on the Hamilton Microlab® STAR™ and 24 samples can be processed in less than 2.5 hours. The system combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with the time-proved efficiency of Omega Bio-tek’s DNA isolation system to provide a fast and convenient method to isolate DNA from a variety of samples. The Mag-Bind® particles provide fast response time when applied to a magnetic field allowing for quick separation from high volumes of liquid.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Product Data

The DNA Yield and Quality Purified from Mag-Bind Blood DNA HV Kit.

Figure 1. DNA Yield & Quality. DNA was extracted from 5mL ad 10 mL of whole blood samples and was eluted in 1 mL and 2 mL volumes. DNA yield and ratios were determined using Thermo Scientific’s NanoDrop™ 2000c. 

The size of purified DNA using Mag-Bind DNA HV Kit is about 60 kb.

Figure 2. TapeStation® Analysis of Purified DNA. TapeStation® analysis of genomic DNA purified from 10 mL of whole blood samples using Omega Bio-tek’s Mag-Bind® Blood DNA HV Kit automated on Hamilton’s Microlab® STAR™. 

Purified DNA using Mag-Bind DNA HV Kit performs successful PCR amplification.

Figure 3. Real-time PCR on Serial Dilutions of Purified DNA. Average Ct values obtained using human-specific primers on 1X, 10X and 100X dilutions of purified DNA used as the template. Sample IDs 1-6 are represented here. 

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