[E-EL-RB0011] Rabbit TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사 Elabscience
납기일 1-2 주
판매가격 823,200원
포인트 0점(구매금액 : 0%)
배송비결제 3,500원   (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

  • [E-EL-RB0011] Rabbit TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit (+0원)

상품 정보

상품 기본설명

96T

상품 상세설명

 

Intended use


This ELISA kit applies to the in vitro quantitative determination of Rabbit TNF-α concentrations in serum, plasma and other biological fluids.

Test principle


This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit TNF-α. You can calculate the concentration of Rabbit TNF-α in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time3.5h
ReactivityRabbit
Detection MethodColormetric
Detection Range15.63—1000 pg/mL
Sensitivity9.38 pg/mL
Sample Volume Required Per Well100μL
Sample TypeSerum, plasma and other biological fluids

Specificity


This kit recognizes Rabbit TNF-α in samples. No significant cross-reactivity or interference between Rabbit TNF-α and analogues was observed.

Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(pg/mL)
O.DAverageCorrected
10002.316
2.364
2.342.281
5001.64
1.676
1.6581.599
2500.921
0.885
0.9030.844
1250.42
0.438
0.4290.37
62.50.269
0.247
0.2580.199
31.250.174
0.148
0.1610.102
15.630.105
0.117
0.1110.052
00.051
0.067
0.059--
SC-E-EL-RB0011.jpg

 

 

 

 

 

 

 

 

 

 

 

 

 

Precision

 

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rabbit TNF-α were tested 20 times
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rabbit TNF-α were tested on 3 different plates, 20 replicates in each plate.

 Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)49.73154.44395.1247.58159.52432.48
Standard deviation2.856.4913.002.458.8722.79
C V (%)5.734.203.295.155.565.27

Recovery


The recovery of Rabbit TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)96-109101
EDTA plasma (n=5)92-10999
Cell culture media (n=5)88-10093

Linearity


Samples were spiked with high concentrations of Rabbit TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

  Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)96-10888-10294-109
Average (%)10294101
1:4Range (%)92-10685-9986-99
Average (%)989193
1:8Range (%)89-10385-9887-99
Average (%)949194
1:16Range (%)90-10580-9286-96
Average (%)978691

Kit components & Storage


An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20°C, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20°C(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8°C(shading light)
Stop Solution1 vial, 10 mL2-8°C
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required


  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37°C
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay Procedure



elisa assay procedure 1 

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations


  1. Hypothermic Machine Perfusion Reduced Inammatory Reaction By Downregulating The Expression Of Matrix Metalloproteinase 9 In A Reperfusion Model Of Donation After Cardiac Death.
    IF: 2.403
    Journal: Artificial Organs(2016)
    DOI: 10.1111/aor.12658
    PMID: 26813475
    Products: E-EL-RB0011 E-EL-RB0014 
    Sample type: Serum
    Reactivity: Rabbit
  2. Hypothermic Machine Perfusion Increases A20 Expression Which Protects Renal Cells Against Ischemia/Reperfusion Injury By Suppressing Inflammation, Apoptosis And Necroptosis.
    IF: 2.341
    Journal: International Journal of Molecular Medicine(2016)
    DOI: 10.3892/ijmm.2016.2586
    PMID: 27177159
    Products: E-EL-RB0011 
    Sample type: Tissue homogenate
    Reactivity: Rabbit
  3. Inhibitory Effects Of S100A4 Gene Silencing On Alkali Burn-Induced Corneal Neovascularization: An In Vivo Study.
    IF: 2.057
    Journal: Molecular Vision(2017)
    PMID: 28479848
    Products: E-EL-RB0011 
    Sample type: Aqueous humor
    Reactivity: Rabbit
  4. Effects Of Local Lipopolysaccharide Administration On The Expression Of Toll-Like Receptor 4 And Pro-Inflammatory Cytokines In Uterus And Oviduct Of Rabbit Does.
    IF: 1.986
    Journal: Theriogenology(2017)
    DOI: 10.1016/j.theriogenology.2017.10.046
    PMID: 29161657
    Products: E-EL-RB0013 E-EL-RB0011 
    Sample type: Plasma
    Reactivity: Rabbit
  5. Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation.
    IF: 2.759
    Journal: Biomedicine & Pharmacotherapy(2018)
    DOI: 10.1016/j.biopha.2017.11.151
    PMID: 29793333
    Products: E-EL-H5518 E-EL-H0755 E-EL-H0149 E-EL-H0109 E-EL-H0102 E-EL-RB0013 E-EL-RB0011 E-EL-RB0014 
    Sample type: Cell culture supernatant,Serum
    Reactivity: Human,Rabbit
  6. Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery.
    IF: 0.629
    Journal: Journal of Obstetrics and Gynaecology(2018)
    DOI: 10.1080/01443615.2017.1400523
    PMID: 29519176
    Products: E-EL-0157 E-EL-RB0011 E-EL-0045 E-EL-RB0014 
    Reactivity: Rabbit
  7. Establishment of a New Zealand White Rabbit Model for Lethal Toxin (LT) Challenge and Efficacy of Monoclonal Antibody 5E11 in the LT-Challenged Rabbit Model.
    IF: 3.273
    Journal: Toxins(2018)
    DOI: 10.3390/toxins10070289
    PMID: 30002351
    Products: E-EL-RB0011 E-EL-RB0013 E-EL-RB0014 E-EL-RB1486 E-EL-RB0679 E-EL-RB0487 
    Sample type: Serum
    Reactivity: Rabbit

 

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