[E-EL-R0015] Rat IL-6(Interleukin 6) ELISA Kit > Biochemical Assay Kits

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[E-EL-R0015] Rat IL-6(Interleukin 6) ELISA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사	Elabscience
납기일	1-2 주
판매가격	693,000원
포인트	0점(구매금액 : 0%)
배송비결제	3,500원 (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

[E-EL-R0015] Rat IL-6(Interleukin 6) ELISA Kit (+0원)
수량

이전상품 [E-EL-R0019] Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit 다음 상품 [E-EL-M2664] Mouse LAMP1(Lysosomal Associated Membrane Protein 1) ELISA Kit

상품 기본설명

96T

상품 상세설명

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat IL-6 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-6. You can calculate the concentration of Rat IL-6 in the samples by comparing the OD of the samples to the standard curve.

Assay type	Sandwich
Format	96T
Assay time	3.5h
Reactivity	Rat
Detection Method	Colormetric
Detection Range	12.50—800 pg/mL
Sensitivity	7.50 pg/mL
Sample Volume Required Per Well	100μL
Sample Type	Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat IL-6 in samples. No significant cross-reactivity or interference between Rat IL-6 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration (pg/mL)	O.D	Average	Corrected
800	2.567 2.418	2.493	2.435
400	1.7 1.628	1.664	1.606
200	0.982 0.971	0.977	0.919
100	0.478 0.512	0.495	0.437
50	0.278 0.271	0.275	0.217
25	0.181 0.184	0.183	0.125
12.50	0.12 0.121	0.121	0.063
0	0.056 0.059	0.058	--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-6 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-6 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	34.58	93.20	396.79	34.39	95.73	379.80
Standard deviation	2.04	4.50	19.96	1.75	4.79	18.61
C V (%)	5.90	4.83	5.03	5.09	5.00	4.90

Recovery

The recovery of Rat IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	98-111	105
EDTA plasma (n=5)	95-109	102
Cell culture media (n=5)	89-95	92

Linearity

Samples were spiked with high concentrations of Rat IL-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	84-94	95-107	96-109
1:2	Average (%)	90	102	101
1:4	Range (%)	93-102	99-108	88-99
1:4	Average (%)	96	103	92
1:8	Range (%)	93-103	89-100	87-101
1:8	Average (%)	100	97	94
1:16	Range (%)	86-96	101-108	83-93
1:16	Average (%)	90	105	90

Kit components & Storage

An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20°C, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20°C(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8°C(shading light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

Microplate reader with 450 nm wavelength filter
High-precision transfer pipette, EP tubes and disposable pipette tips
Incubator capable of maintaining 37°C
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Assay Procedure

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results