[E-EL-H1360] Human PPAR-α(Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사 Elabscience
납기일 1-2 주
판매가격 852,600원
포인트 0점(구매금액 : 0%)
배송비결제 3,500원   (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

  • [E-EL-H1360] Human PPAR-α(Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit (+0원)

상품 정보

상품 기본설명

96T

상품 상세설명

 

Intended use


This ELISA kit applies to the in vitro quantitative determination of Human PPAR-α concentrations in serum, plasma and other biological fluids.

Test principle


This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PPAR-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPAR-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PPAR-α. You can calculate the concentration of Human PPAR-α in the samples by comparing the OD of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time3.5h
ReactivityHuman
Detection MethodColormetric
Detection Range0.16—10 ng/mL
Sensitivity0.10 ng/mL
Sample Volume Required Per Well100μL
Sample TypeSerum, plasma and other biological fluids

Specificity


This kit recognizes Human PPAR-α in samples. No significant cross-reactivity or interference between Human PPAR-α and analogues was observed.

Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(ng/mL)
O.DAverageCorrected
102.44
2.454
2.4472.393
51.533
1.583
1.5581.504
2.50.891
0.887
0.8890.835
1.250.373
0.397
0.3850.331
0.630.258
0.254
0.2560.202
0.320.156
0.148
0.1520.098
0.160.103
0.107
0.1050.051
00.052
0.056
0.054--
SC-E-EL-H1360.jpg

 

 

 

 

 

 

 

 

 

 

 

Precision

 

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PPAR-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PPAR-α were tested on 3 different plates, 20 replicates in each plate.

 Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (ng/mL)0.491.483.430.491.393.24
Standard deviation0.030.060.180.020.060.14
C V (%)6.124.055.254.084.324.32

Recovery


The recovery of Human PPAR-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)89-10295
EDTA plasma (n=5)89-10496
Cell culture media (n=5)95-108100

Linearity


Samples were spiked with high concentrations of Human PPAR-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

  Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)92-10391-10687-104
Average (%)979995
1:4Range (%)91-10585-9786-98
Average (%)989093
1:8Range (%)93-10482-9385-98
Average (%)988691
1:16Range (%)87-9985-9886-98
Average (%)949292

Kit components & Storage


An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20°C, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20°C(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8°C(shading light)
Stop Solution1 vial, 10 mL2-8°C
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required


  • Microplate reader with 450 nm wavelength filter
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37°C
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay Procedure



elisa assay procedure 1 

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

 

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