Product introduction
MMC-6HP is a kind of multi-mode salt tolerant biological separation medium, which is suitable for group-separation and moderate purification of all types of charged biomolecules, such as proteins, peptides, nucleic acids, etc.
Figure 1: The ligand of MMC-6HP is a kind of multi-mode ligand, it has many types of interaction with target molecules, mainly include the ionic interaction, and followed by hydrogen binding and hydrophobic interaction, etc.
Advantages
1. Combine with charged molecules under high conductance conditions. The sample is no longer restricted by electrical conductivity and can processed ion exchange without pretreatment.
2. High binding capacity. It has a higher processing capacity under flowthrough mode.
3. It has a new selectivity compared with the traditional ion exchange media.
4. High resolution.
Performance index
Matrix | Highly cross-linked 6% agarose |
Particle size range | 25-45 µm |
Average particle size | 37 µm |
Media type | Weak cation exchanger |
Ionic capacity | 120-180 µmol H+/mL (media) |
Binding capacity | ≥60 mg BSA/mL (media) |
pH stability | 2-14 (short term) 2-12 (working) 2-12 (long term) |
Chemical stability | All of the commonly used buffers, 1M NaOH, 70% Ethanol, 8M urea, 6M Guanidine hydrochloride. Avoid of oxidizing agent, cationic detergents and cationic buffer |
Flow rate | 150-250 cm/h (0.3MPa, XK16/40. Column bed height:30 cm) |
Pressure | ≤ 0.3 MPa |
Storage buffer | 20% Ethanol |
Storage temperature | 4~30℃ |
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