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Product introduction
MMA-6FF is a kind of multi-mode biological separation medium, which is mainly used for moderate and fine purification of monoclonal antibody (to remove the Protein A* after Protein A purification, dimers, polymers, host cell proteins, nucleic acids), and it can also be used for fine purification of other biological molecules (to remove dimers, polymers, host cell proteins, nucleic acids, etc).
* Micro amount of Protein A may drop off in the process of operating Protein A-4HF.
Figure 1: The ligand of MMA-6FF is a kind of multi-mode ligand, it has many types of interaction with target molecules, mainly include the ionic interaction (strong anion interaction), and followed by hydrogen binding and hydrophobic interaction, etc.
Advantages
1. Combine with charged molecules under high conductance conditions. The sample is no longer restricted by electrical conductivity and can processed ion exchange without pretreatment.
2. High binding capacity. It has a higher processing capacity under flowthrough mode.
3. It has a new selectivity compared with the traditional ion exchange media.
Performance index
Matrix | Highly cross-linked 6% agarose |
Particle size range | 45-165 µm |
Average particle size | 90 µm |
Media type | Strong anion exchanger |
Ionic capacity | 90-130 µmol Cl-/mL (media) |
Binding capacity | ≥75mg BSA/mL (media) |
pH stability | 2-14 (short term) 3-12 (working) 3-12 (long term) |
Chemical stability | All of the commonly used buffers, 1M acetate, 1M NaOH, 70% Ethanol, 8M urea, 6M Guanidine hydrochloride. Avoid of oxidizing agent and anionic detergent |
Flow rate | 300-600 cm/h (0.3MPa, XK16/40. Column bed height:30 cm) |
Pressure | ≤ 0.3 MPa |
Storage buffer | 20% Ethanol |
Storage temperature | 4~30℃ |
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