Product introduction
DEAF-6FF is suitable for separation and purification of all types of charged biomolecules, such as proteins, peptides, nucleic acids, etc.
Advantages
1. Quick, easy, convenient.
2. Wide application range. It is suitable for component separation or fine purification of all types of charged biomolecules.
3. The purification process is highly flexible, and the purity of the sample can be improved by screening the purification conditions in earlier stage.
4. High binding capacity (compared with other types of chromatographic media).
Performance index
Ligand | Diethylaminoethyl |
Matrix | Highly cross-linked 6% agarose |
Particle size range | 45-165 µm |
Average particle size | 90 µm |
Media type | Weak anion exchanger |
Charged group | -N+ H2(C2H5)2 |
Ionic capacity | 150-300 µmol Cl-/mL (media) |
Binding capacity | 110 mg BSA/mL (media) |
pH stability | 1-14 (short term) 2-9 (working) 2-12 (long term) |
Chemical stability | All of the commonly used buffers, 1M NaOH, 70% Ethanol, 8M urea, 6M Guanidine hydrochloride. Avoid of oxidizing agent, anionic detergent and anionic buffer |
Flow rate | 200-300 cm/h (0.3MPa, XK16/40. Column bed height:30 cm) |
Pressure | ≤0.3 MPa |
Storage buffer | 20% Ethanol |
Storage temperature | 4~30℃ |
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