Product introduction
EAH-6FF is a kind of fast flow purification media which was prepared by covalently coupling the ethylenediamine to agarose matrix. It contains an amino group at the end of the spacer, and is mainly suitable for coupling of small molecular compounds (ligand) containing free carboxyl.
The coupling should be reacted under acidic conditions (acid catalysis). Addition of coupling reagent (carbodiimide, such as EDC, CMC) can promote the condensation of free amino and free carboxyl.
Advantages
1. Wide application range: It can be used for coupling of macro biomolecules containing amino groups.
2. Multi-point coupling. Simple, flexible, fast and effective. It can efficiently maintain the biological activity and stability of biomolecules.
3. Fast flow rate, high yields and easy to be implemented.
Performance index
Matrix | Highly cross-linked 6% agarose |
Particle size range | 45-165 µm |
Average particle size | 90 µm |
Active group | Amino |
Ligand density* | 0.4-0.5 mM Cl-/mL (media) |
Spacer arm | 15 atoms |
Coupling temperature | 4-25℃ |
Coupling pH | 4.5-6.5 |
pH stability | 3-14 |
Chemical stability | All commonly used buffers |
Flow rate | 250-600 cm/h (0.3MPa, XK16/40. Column bed height: 30 cm) |
Storage buffer | 20% Ethanol containing 0.9% NaCl |
Storage temperature | 4~8℃ |
*: The ligand density depends on the type of ligand and coupling condition.
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