[E-CL-M0393] Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) CLIA Kit > Biochemical Assay Kits

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[E-CL-M0393] Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) CLIA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사	Elabscience
납기일	2-3 일
판매가격	954,800원
포인트	0점(구매금액 : 0%)
배송비결제	3,500원 (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

[E-CL-M0393] Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) CLIA Kit (+0원)
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이전상품 [E-CL-M0394] Mouse RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit 다음 상품 [E-CL-M0392] Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) CLIA Kit

상품 기본설명

96T

상품 상세설명

Intended use

This CLIA kit applies to the in vitro quantitative determination of Mouse TNFRSF1B concentrations in serum, plasma and other biological fluids.

Test principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF1B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNFRSF1B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNFRSF1B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TNFRSF1B. You can calculate the concentration of Mouse TNFRSF1B in the samples by comparing the RLU value of the samples to the standard curve.

Assay type	Sandwich
Format	96T
Assay time	3.5h
Reactivity	Mouse
Detection method	Chemiluminescence
Detection range	3.13-200 pg/mL
Sensitivity	1.88 pg/mL
Sample volume	100μL
Sample type	Serum, plasma and other biological fluids
Repeatability	CV < 15%

Specificity

This kit recognizes Mouse TNFRSF1B in samples. No significant cross-reactivity or interference between Mouse TNFRSF1B and analogues was observed.

Typical data

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration (pg/mL)	RLU	Average	Corrected
200	41891 46255	44073	44035
100	17940 18482	18211	18173
50	8282 8130	8206	8168
25	3843 4027	3935	3897
12.5	1988 1976	1982	1944
6.25	1064 1040	1052	1014
3.13	594 602	598	560
0	38 38	38	--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TNFRSF1B were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TNFRSF1B were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	10.73	20.00	91.14	9.99	21.21	94.57
Standard deviation	1.34	1.47	7.34	1.22	2.08	8.10
C V (%)	12.49	7.35	8.05	12.21	9.81	8.57

Recovery

The recovery of Mouse TNFRSF1B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	88-98	93
EDTA plasma (n=5)	97-112	104
Cell culture media (n=5)	103-117	108

Linearity

Samples were spiked with high concentrations of Mouse TNFRSF1B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-110	89-101	95-107
1:2	Average (%)	102	94	101
1:4	Range (%)	90-105	91-109	85-99
1:4	Average (%)	96	99	91
1:8	Range (%)	97-110	95-110	89-100
1:8	Average (%)	103	101	95
1:16	Range (%)	96-109	100-112	94-109
1:16	Average (%)	102	105	101

Kit components& Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	2-8℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (shading light)
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

Chemiluminescence immunoassay analyzer
High-precision transfer pipette, EP tubes and disposable pipette tips
Incubator capable of maintaining 37℃
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Assay procedure

	1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.
	2.Remove the liquid.
	3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.
	4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.
	5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.
	6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.