[E-CL-M0094] Mouse NRG-4 (Neuregulin 4) CLIA Kit > Biochemical Assay Kits

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[E-CL-M0094] Mouse NRG-4 (Neuregulin 4) CLIA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사	Elabscience
납기일	1-2 주
판매가격	954,800원
포인트	0점(구매금액 : 0%)
배송비결제	3,500원 (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

[E-CL-M0094] Mouse NRG-4 (Neuregulin 4) CLIA Kit (+0원)
수량

이전상품 [E-CL-M0101] Mouse APOE(Apolipoprotein E) CLIA Kit 다음 상품 [E-CL-H1281] Human VEGF165 (Vascular Endothelial Growth Factor165) CLIA Kit

상품 기본설명

96T

상품 상세설명

Intended use

This CLIA kit applies to the in vitro quantitative determination of Mouse NRG-4 concentrations in serum, plasma and other biological fluids.

Test principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NRG-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NRG-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NRG-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NRG-4. You can calculate the concentration of Mouse NRG-4 in the samples by comparing the RLU value of the samples to the standard curve.

Assay type	Sandwich
Format	96T
Assay time	3.5h
Reactivity	Mouse
Detection method	Chemiluminescence
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample volume	100μL
Sample type	Serum, plasma and other biological fluids
Repeatability	CV < 15%

Specificity

This kit recognizes Mouse NRG-4 in samples. No significant cross-reactivity or interference between Mouse NRG-4 and analogues was observed.

Typical data

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration (pg/mL)	RLU	Average	Corrected
4000	57779 60383	59081	59046
2000	25135 30583	27859	27824
1000	14056 12912	13484	13449
500	6366 6844	6605	6570
250	3292 3194	3243	3208
125	1684 1478	1581	1546
62.50	747 763	755	720
0	35 35	35	--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NRG-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NRG-4 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	189.82	477.76	1746.17	172.80	442.72	1684.39
Standard deviation	17.06	53.17	114.72	21.46	50.56	117.07
C V (%)	8.99	11.13	6.57	12.42	11.42	6.95

Recovery

The recovery of Mouse NRG-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	92-105	97
EDTA plasma (n=5)	92-105	97
Cell culture media (n=5)	95-109	103

Linearity

Samples were spiked with high concentrations of Mouse NRG-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	92-104	94-105	97-108
1:2	Average (%)	97	99	102
1:4	Range (%)	103-120	99-114	88-99
1:4	Average (%)	110	107	93
1:8	Range (%)	90-102	88-104	86-100
1:8	Average (%)	95	95	92
1:16	Range (%)	86-97	91-103	90-103
1:16	Average (%)	91	96	96

Kit components& Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	2-8℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (shading light)
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

Chemiluminescence immunoassay analyzer
High-precision transfer pipette, EP tubes and disposable pipette tips
Incubator capable of maintaining 37℃
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Assay procedure

	1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.
	2.Remove the liquid.
	3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.
	4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.
	5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.
	6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.