[E-CL-M0094] Mouse NRG-4 (Neuregulin 4) CLIA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사 Elabscience
납기일 1-2 주
판매가격 954,800원
포인트 0점(구매금액 : 0%)
배송비결제 3,500원   (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

  • [E-CL-M0094] Mouse NRG-4 (Neuregulin 4) CLIA Kit (+0원)

상품 정보

상품 기본설명

96T

상품 상세설명

 

Intended use


This CLIA kit applies to the in vitro quantitative determination of Mouse NRG-4 concentrations in serum, plasma and other biological fluids.

Test principle


This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NRG-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NRG-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NRG-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NRG-4. You can calculate the concentration of Mouse NRG-4 in the samples by comparing the RLU value of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time3.5h
ReactivityMouse
Detection methodChemiluminescence
Detection range62.50-4000 pg/mL
Sensitivity37.50 pg/mL
Sample volume100μL
Sample typeSerum, plasma and other biological fluids
RepeatabilityCV < 15%

Specificity


This kit recognizes Mouse NRG-4 in samples. No significant cross-reactivity or interference between Mouse NRG-4 and analogues was observed.

Typical data


As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(pg/mL)
RLUAverageCorrected
400057779
60383
5908159046
200025135
30583
2785927824
100014056
12912
1348413449
5006366
6844
66056570
2503292
3194
32433208
1251684
1478
15811546
62.50747
763
755720
035
35
35--


 

 

 

 

 

 

 

 

 

 

 

Precision

 

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NRG-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NRG-4 were tested on 3 different plates, 20 replicates in each plate.

 Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)189.82477.761746.17172.80442.721684.39
Standard deviation17.0653.17114.7221.4650.56117.07
C V (%)8.9911.136.5712.4211.426.95

Recovery


The recovery of Mouse NRG-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)92-10597
EDTA plasma (n=5)92-10597
Cell culture media (n=5)95-109103

Linearity


Samples were spiked with high concentrations of Mouse NRG-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

  Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)92-10494-10597-108
Average (%)9799102
1:4Range (%)103-12099-11488-99
Average (%)11010793
1:8Range (%)90-10288-10486-100
Average (%)959592
1:16Range (%)86-9791-10390-103
Average (%)919696

Kit components& Storage


An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro CLIA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent A1 vial, 5 mL2-8℃ (shading light)
Substrate Reagent B1 vial, 5 mL2-8℃ (shading light)
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required


  • Chemiluminescence immunoassay analyzer
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37℃
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay procedure



elisa assay procedure 1 

1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.

elisa assay procedure 2lct_08.png

2.Remove the liquid.

elisa assay procedure 3lct_08.png

3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.

elisa assay procedure 4lct_08.png

4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.

elisa assay procedure 5lct_08.png

5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.

elisa assay procedure 6lct_08.png

6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.

 

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