[E-CL-H0997] Human HMGB-1(High Mobility Group Protein B1) CLIA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사 Elabscience
납기일 2-3 일
판매가격 954,800원
포인트 0점(구매금액 : 0%)
배송비결제 3,500원   (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

  • [E-CL-H0997] Human HMGB-1(High Mobility Group Protein B1) CLIA Kit (+0원)

상품 정보

상품 기본설명

96T

상품 상세설명

 

Intended use


This CLIA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.

Test principle


This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.

Assay typeSandwich
Format96T
Assay time3.5h
ReactivityHuman
Detection methodChemiluminescence
Detection range12.50-800 pg/mL
Sensitivity7.50 pg/mL
Sample volume100μL
Sample typeSerum, plasma and other biological fluids
RepeatabilityCV < 15%

Specificity


This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.

Typical data


As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration
(pg/mL)
RLUAverageCorrected
80050035
55249
5264252616
40021479
21989
2173421708
20010340
9076
97089682
1004353
4751
45524526
502204
2172
21882162
251140
980
10601034
12.50474
544
509483
025
27
26--
SC-E-CL-H0997.jpg

 

 

 

 

 

 

 

 

 

 

 

 

Precision

 

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HMGB-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HMGB-1 were tested on 3 different plates, 20 replicates in each plate.

 Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)38.9471.20397.7937.0072.83379.26
Standard deviation4.748.1627.923.485.4828.71
C V (%)12.1711.467.029.417.527.57

Recovery


The recovery of Human HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)99-111104
EDTA plasma (n=5)94-111102
Cell culture media (n=5)86-9991

Linearity


Samples were spiked with high concentrations of Human HMGB-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

  Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)93-109103-118103-120
Average (%)101109109
1:4Range (%)95-11194-10785-98
Average (%)1039990
1:8Range (%)93-10986-100101-114
Average (%)9993108
1:16Range (%)88-10185-9988-101
Average (%)959194

Kit components& Storage


An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro CLIA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent A1 vial, 5 mL2-8℃ (shading light)
Substrate Reagent B1 vial, 5 mL2-8℃ (shading light)
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required


  • Chemiluminescence immunoassay analyzer
  • High-precision transfer pipette, EP tubes and disposable pipette tips
  • Incubator capable of maintaining 37℃
  • Deionized or distilled water
  • Absorbent paper
  • Loading slot for Wash Buffer

Assay procedure



elisa assay procedure 1 

1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.

elisa assay procedure 2lct_08.png

2.Remove the liquid.

elisa assay procedure 3lct_08.png

3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.

elisa assay procedure 4lct_08.png

4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.

elisa assay procedure 5lct_08.png

5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.

elisa assay procedure 6lct_08.png

6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.

 

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