[E-CL-H0997] Human HMGB-1(High Mobility Group Protein B1) CLIA Kit > Biochemical Assay Kits

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[E-CL-H0997] Human HMGB-1(High Mobility Group Protein B1) CLIA Kit 요약정보 및 구매

96T

상품 선택옵션 0 개, 추가옵션 0 개

제조사	Elabscience
납기일	2-3 일
판매가격	954,800원
포인트	0점(구매금액 : 0%)
배송비결제	3,500원 (주문금액 10만원 이상일시 배송비 무료)

선택된 옵션

[E-CL-H0997] Human HMGB-1(High Mobility Group Protein B1) CLIA Kit (+0원)
수량

이전상품 [E-CL-H0998] Human PGF (Placental Growth Factor) CLIA Kit 다음 상품 [E-CL-H0993] Human PD-L1(Programmed Cell Death Protein 1 Ligand 1) CLIA Kit

상품 기본설명

96T

상품 상세설명

Intended use

This CLIA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.

Test principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.

Assay type	Sandwich
Format	96T
Assay time	3.5h
Reactivity	Human
Detection method	Chemiluminescence
Detection range	12.50-800 pg/mL
Sensitivity	7.50 pg/mL
Sample volume	100μL
Sample type	Serum, plasma and other biological fluids
Repeatability	CV < 15%

Specificity

This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.

Typical data

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration (pg/mL)	RLU	Average	Corrected
800	50035 55249	52642	52616
400	21479 21989	21734	21708
200	10340 9076	9708	9682
100	4353 4751	4552	4526
50	2204 2172	2188	2162
25	1140 980	1060	1034
12.50	474 544	509	483
0	25 27	26	--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HMGB-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HMGB-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	38.94	71.20	397.79	37.00	72.83	379.26
Standard deviation	4.74	8.16	27.92	3.48	5.48	28.71
C V (%)	12.17	11.46	7.02	9.41	7.52	7.57

Recovery

The recovery of Human HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	99-111	104
EDTA plasma (n=5)	94-111	102
Cell culture media (n=5)	86-99	91

Linearity

Samples were spiked with high concentrations of Human HMGB-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	93-109	103-118	103-120
1:2	Average (%)	101	109	109
1:4	Range (%)	95-111	94-107	85-98
1:4	Average (%)	103	99	90
1:8	Range (%)	93-109	86-100	101-114
1:8	Average (%)	99	93	108
1:16	Range (%)	88-101	85-99	88-101
1:16	Average (%)	95	91	94

Kit components& Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	2-8℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (shading light)
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

Chemiluminescence immunoassay analyzer
High-precision transfer pipette, EP tubes and disposable pipette tips
Incubator capable of maintaining 37℃
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Assay procedure

	1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.
	2.Remove the liquid.
	3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.
	4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.
	5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.
	6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.