Application

This kit is used for quantitative determination of α- hydroxybutyrate dehydrogenase (HBDH) in serum.

Detection significance

The increase of HBDH normally occur with myocardial infarction, active rheumatic myocarditis, hemolytic anemia and other diseases.

Detection principle

HBDH activity can be calculated by measuring the decrease rate of NADH at 340nm.

Storage and validity period

The valid of kit is 12 months when stored at 2℃~8℃ in the dark. It is stable for 1 month when stored at 2℃~8℃ in the dark after opening. Avoid freezing.

Sample requirements

Serum should be separated immediately after collecting blood.

Operation steps

1.  Operate with automatic biochemical analyzer

2.  Operate with ultraviolet spectrophotometer

Reference range

72~182 U/L (It is recommended to establish your own laboratory reference range).

Performance index

1.  The absorbance of blank: A340nm (1.0 cm) ≥ 1.0.

2.  The change rate of absorbance of blank: △A/min ≤ 0.005.

3.  Linearity range: 0~1200 U/L, r2 ≥ 0.99.

4.  Accuracy: Relative deviation ≤ 15%.

5.  Precision: intra-assay CV≤ 5%, inter-assay CV≤ 5%.

Notes

1.  This product is for scientific research use only, not for clinical diagnosis.

2.  Avoid to contact the reagent with skin and clothing. Wash immediately with plenty of water if contact it carelessly.

3.  Instructions should be followed strictly, changes of operation may result in unreliable results.

4.  The amount of reagent and sample can be increased and decreased proportionately

5.  The ratio of sample and reagent can be scaled as required.

6.  Do not use components from different batches of kit.

7.  Please take safety precautions and follow the procedures of laboratory reagent operation. All waste liquid should be handled in accordance with local regulations.